The HAMBURG SCHOOL OF FOOD SCIENCE (HSFS) was founded in 2011 by Markus Fischer. The orientation of the HSFS is interdisciplinary and is characterized by the combination of classical food chemistry questions with modern biochemical / nutritional diagnostics. In the food sector, we mainly work on the development of analytical methods for the authenticity and quality control of food. In addition to methods for “in-field” analysis, we are interested in methods for routine applications, as well as technically very sophisticated fingerprinting methods.
A number of research projects are currently being carried out at the HSFS which are funded through the AiF / FEI or the BLE (Federal Institute for Agriculture and Food) within the framework of the program for the promotion of the IGF (Industrial Community Research) by the Federal Ministry of Economics and Energy (BMWi) the BMEL (Federal Ministry of Food and Agriculture).
Operationally carried out at the HSFS are analyzes of the underlying genetic information (food GENOMICS) and the non-volatile metabolom (food METABOLOMICS). Hereinafter, by means of ultra-high-resolution technologies (for example NGS methods, HR-LC-MS), hypothesis-free system-wide statements about the biochemical processes occurring in a foodstuff are also made accessible with regard to interactions with the environment (FOOD FINGERPRINTING). First of all, profiles (maxi-fingerprints) are collected and compared with each other using multivariate static methods from all addressed authentic raw materials in infrastructurally sophisticated experimental approaches. The stable molecular differences identified in this way between the reference data sets are both qualitatively and quantitatively characteristic of the respective sample. For a later assignment it is sufficient to determine these minifingerprints by technically less complex, routine targeted analyzes (FOOD TARGETING). In addition, it is possible to determine individual chemical markers qualitatively (FOOD SENSING) by means of a rapid test procedure. The CNFP covers the areas of FOOD FINGERPRINTING and FOOD TARGETING.
In addition to the composition of foodstuffs and raw materials, the question of the effect of food plays an increasing role at the HSFS. Strategies for METABOLIC PROFILING and the targeted recording of nutrition-relevant parameters (METABOLIC TARGETING) using high-resolution spectroscopic procedures are at the center of research activities at the HSFS.
References to specific research topics:
AUTHENTICATION – OVERVIEW
Omics-Approaches for Food Authentication
M. Creydt, M. Fischer
Electrophoresis 2018 Jul;39(13):1569-1581 (2018)
Development of a multiplex real-time PCR for determination of apricot in marzipan using the PLEXOR® System
Schelm, S.; Haase, I.; Fischer, C., Fischer, M.
Journal of Agricultural and Food Chemistry 65, 516-522 (2017)
Applying Population Genetics for Authentication of Marine Fish: The Case of Saithe (Pollachius virens)
K. Behrmann, H. Rehbein, A. von Appen, M. Fischer
Journal of Agricultural and Food Chemistry 63, 802-809 (2015)
Food Fingerprinting: Characterization of the Ecuadorean Type CCN-51 of Theobroma cacao L. using microsatellite markers
L. Herrmann, C. Felbinger, I. Haase, B. Rudolph, B. Biermann, M. Fischer
Journal of Agricultural and Food Chemistry 63, 4539-4544 (2015)
DNA-based Differentiation of the Ecuadorian Cocoa Types CCN-51 and Arriba Based on Sequence Differences in the Chloroplast Genome
L. Herrmann, I. Haase, M. Blauhut, N. Barz, M. Fischer
Journal of Agricultural and Food Chemistry 62, 12118-12127 (2014)
FOOD & HEALTH Metabolomics
Plant Metabolomics – Maximizing Metabolome Coverage by Optimizing Mobile Phase Additives for Non-Targeted Mass Spectrometry in Positive and Negative Electrospray Ionization Mode
Marina Creydt, Markus Fischer
Analytical Chemistry (in press) (2017)
Food Targeting: Geographical origin determination of hazelnuts (Corylus avellana) by LC-QqQ-MS/MS based targeted metabolomics application.
Klockmann S, Reiner E, Cain N, Fischer M.
Journal of Agricultural and Food Chemistry 65, 1456-1465 (2017)
Cold-induced conversion of cholesterol to bile acids in mice shapes the gut microbiome and promotes adaptive thermogenesis
A. Worthmann, C. John, M.C. Rühlemann, M. Baguhl, F.-A. Heinsen, N. Schaltenberg, M. Heine, C. Schlein, I. Evangelakos, C. Mineo, M. Fischer, M. Dandri, C. Kremoser, L. Scheja, A. Franke, P. W. Shaul, J. Heeren
Nat Med. 2017 Jun 12. doi: 10.1038/nm.4357. [Epub ahead of print] (2017)
Thermogenic adipocytes promote HDL turnover and reverse cholesterol transport
A. Bartelt, C. John, N. Schaltenberg, J. Berbée, A. Worthmann, M. L. Cherradi, C. Schlein, J. Schmidt, M. Boon, F. Rinninger, M. Heine, K. Toedter, S. K Nilsson, M. Fischer, A. Niemeier, S. Wijers, W. v. Marken Lichtenbelt, L. Scheja, P. Rensen, J. Heeren
Nature Communications 8, 15010 (2017)
Metabolite Profiling: Development and Application of an UHR-QTOF-MS(/MS) method approach for the assessment of metabolic changes in high fat diet fed mice
P. Werner, E. Meiss, L. Scheja, J. Heeren, M. Fischer
Metabolomics 13, 44 (10 pp) (2017)
Food Fingerprinting: Metabolomic approaches for geographical origin discrimination of hazelnuts (Corylus avellana) by UPLC-QTOF-MS
Klockmann, S.; Reiner, E.; Bachmann, R.; Hackl, T., Fischer, M.
Journal of Agricultural and Food Chemistry 64, 9253-9262 (2016)
Metabolite targeting: Development of a Comprehensive Targeted Metabolomics Platform for the Assessment of Diabetes and its Complications
E. Meiss, P. Werner, C. John, L. Scheja, N. Herbach, J. Heeren, M. Fischer
Metabolomics 12, 52 (2016
Aptamers: Universal capture units for lateral flow applications
C. Fischer, H. Wessels, A. Paschke-Kratzin, M. Fischer
Analytical Biochemistry 522, 53-60 (2017)
Automatized Enrichment of Sulfanilamide in Milk Matrices by Utilization of Aptamer Linked Magnetic Particles
C. Fischer, C. Kallinich, S. Klockmann, J. Schrader, M. Fischer
Journal of Agricultural and Food Chemistry 64, 9246-9252 (2016)
Food Sensing: Selection and Characterization of DNA Aptamers to Alicyclobacillus Spores for Trapping and Detection from Orange Juice
T. Hünniger, C. Fischer, H. Wessels, A. Hoffmann, A. Paschke-Kratzin, I. Haase, M. Fischer
Journal of Agricultural and Food Chemistry 63, 2189-2197 (2015)
just in time-Selection: A rapid semi-automated SELEX of DNA aptamers using magnetic separation and BEAMing
T. Hünniger, H. Wessels, C. Fischer, A. Paschke-Kratzin, M. Fischer
Analytical Chemistry 86, 10940-10947 (2014)
Prof. Dr. Markus Fischer
Mathematics, Informatics and Natural Sciences (MIN)
Department of Chemistry
HAMBURG SCHOOL OF FOOD SCIENCE – Institute of Food Chemistry
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